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Fiber Optic Oxygen Sensors Neofox FOXY HIOXY and PHOSPOR
Spectrecology sells sensors based on the finest materials in the world. Our sol-gel sensor materials are the culmination of over 15 years of R&D effort. No one makes fluorescent sensors with the stability and durability of our FOXY line. Originally developed for oceanographic use, these sensors are deployed in aircraft fuel tanks, bioreactors, chemical reactors, respitory therapy devices, food and pharmaceutical packaging plants, brain tisse, soil, tree sap and even CO2 lasers.
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Fiber Optic Oxygen Sensors consist of an optical fiber polished to an angled surface and coated with a thin layer of sol-gel containing the active fluorophore. The probes can be made in many styles, depending on the requirement for size, ruggedness etc. If you don't find a style that meets your needs, ask us. We can easily build custome versions.
Fiber Optic Oxygen Sensors are designed for use with our modular components. First select a Sensor Coating Formulation. Then select a Fiber Optic Probe onto which the sensor coating will be applied. The select an optical fiber and the NEOFOX electronics package of your choice. Need help? Just call us and we'll pick the best components for you. |
The tables below list various off-the-shelf probes and the reccomended bifurcated fiber assembly.
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Our standard lab probe is called the R probe. It is a 1/16" OD stainless steel ferrule with a 1000 um fiber.
| Probe Assembly: | 1000 µm fiber in a stainless steel 1/16" OD ferrule |
| Dimensions: | 1.587 mm OD, 152.4 mm length |
| Pressure: | 300 psi |
| Fiber: | BIFBORO-1000-2 |
| Termination: | SMA 905 |
| Typical Usage: | general purpose |
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AL300The AL300 is a 500 µm OD (300 µm core diameter) aluminum-jacketed optical fiber probe for applications that require fine spatial resolution.
| Probe Assembly: | 300 µm aluminum-jacketed fiber assembly |
| Dimensions: | 500 µm OD, 1 m length |
| Pressure: | 300 psi |
| Fiber: | BIFBORO-300-2 |
| Termination: | SMA 905 |
| Typical Usage: | fine spatial resolution applications |
| Price: | $577 |
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18GThe 18G is a 300 µm optical fiber housed in an 18-gauge stainless steel needle probe.
*Refurbishing not available on this item
| Probe Assembly: | 300 µm optical fiber, 18-gauge tip |
| Dimensions: | 1.27 mm diameter, 90 mm length tip |
| Pressure: | 300 psi |
| Fiber: | BIFBORO-300-2 |
| Termination: | SMA 905 |
| Typical Usage: | penetration of vial septa and rigid packaging |
| Price: | $692 |
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21GThe 21G is a 300 µm optical fiber housed in a 21-gauge stainless steel needle probe.
*Refurbishing not available on this item
| Probe Assembly: | 300 µm optical fiber, 21-gauge tip |
| Dimensions: | 813 µm diameter, 90 mm length tip |
| Pressure: | 300 psi |
| Fiber: | BIFBORO-300-2 |
| Termination: | SMA 905 |
| Typical Usage: | penetration of soft packaging |
| Price: | $692 |
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OR125The OR125 is a 1/8" OD optical fiber probe used as a direct replacement for 1/8" OD O2 electrodes.
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Probe Assembly: |
1000 µm optical fiber, stainless steel ferrule |
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Dimensions: |
3.175 mm OD, 63.5 mm length |
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Pressure: |
300 psi |
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Fiber: |
BIFBORO-1000-2 |
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Termination: |
SMA 905 |
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Typical Usage: |
direct replacement for 1/8" OD O2 electrodes |
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Price: |
$692 |
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OR125GThe OR125 is a 1/8" OD optical fiber probe used as a direct replacement for 1/8" OD O2 electrodes.
| Probe Assembly: | 1000 µm optical fiber, stainless steel ferrule |
| Dimensions: | 3.175 mm OD, 63.5 mm length |
| Pressure: | 300 psi |
| Fiber: | BIFBORO-1000-2 |
| Termination: | SMA 905 |
| Typical Usage: | direct replacement for O-ring grooved electrodes |
| Price: | $692 |
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OR125GTThe OR125 is a 1/8" OD optical fiber probe used as a direct replacement for 1/8" OD O2 electrodes.
| Probe Assembly: | 1000 µm optical fiber, titanium ferrule |
| Dimensions: | 3.175 mm OD, 63.5 mm length |
| Pressure: | 300 psi |
| Fiber: | BIFBORO-1000-2 |
| Termination: | SMA 905 |
| Typical Usage: | direct replacement for O-ring grooved electrodes |
| Price: | $751 |
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T1000The T1000 is a 6.35 mm OD optical fiber probe used in process environments and high-pressure applications.
| Probe Assembly: | 1000 µm optical fiber, stainless steel ferrule |
| Dimensions: | 6.35 mm OD, 177.8 mm length |
| Pressure: | 3000 psi |
| Fiber: | BIFBORO-1000-2 |
| Termination: | SMA 905 |
| Typical Usage: | process environments, high-pressure applications |
| Price: | $1154 |
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T1000-RTDThe T1000-RTD is a 6.35-mm OD optical fiber probe with an embedded RTD used in process environments. The T1000 RTD can only be used in conjunction with the USB-LS-450.
| Probe Assembly: | 1000 µm optical fiber, 1/8" OD stainless steel |
| Dimensions: | 6.355 mm OD, 177.8 mm length |
| Pressure: | 300 psi |
| Fiber: | BIFBORO-1000-2 |
| Termination: | SMA 905 |
| Typical Usage: | process environments, includes embedded RTD |
| Price: | $1732 |
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PI600The PI600 is a silicone-jacketed, polyimide-coated optical fiber probe used in environments where a non-metallic probe is required.
| Probe Assembly: | 600 µm optical fiber with silicone jacketing* |
| Dimensions: | 710 µm OD, 2 m length |
| Pressure: | 300 psi |
| Fiber: | BIFBORO-600-2 |
| Termination: | SMA 905 |
| Typical Usage: | environments where non-metallic probe is indicated |
| Price: | $577 |
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Optional coatings of silicone (both standard and medical-grade) can be applied to Probes to exclude ambient light, improve chemical resistance and eliminate refractive index effects. We recommend an overcoat if your application involves liquids, as well as those that involve changing from gases to liquids, as scattering effects could result in the sensor losing calibration. There is no charge for these overcoats.
| Item | Description | Response in Gases | Response in Liquids |
| -AF | RTV silicone adhesive overcoat (increases probe response time) | 10-30 seconds | 15-45 seconds |
| -AF-MG | High-strength RTV silicone adhesive overcoat is a medical implant-grade silicone | 15-45 seconds | 45-60 seconds (depending on sample viscosity) |

Remote Detection! -- That's right! We can sense the fluorescent decay time from a distance. Our Red Eye patches can be put on the inside of package, vial, respirometer, you name it. Point the fiber optic probe at the patch and get a fast accurate answer.
For details on O2 sensing patches, click here!
Our Oxygen Sensing Probes with the FOXY Formulation applied are designed for monitoring partial pressure of oxygen in benign gasses and aqueous liquids. Standard FOXY probe tips are covered with a layer of hydrophobic sol-gel material with a ruthenium compound trapped in the sol-gel matrix. When excited by an LED, the ruthenium complex fluoresces. If the excited ruthenium complex then encounters an oxygen molecule, the excess energy quenches the fluorescent signal. The fluorescence intensity or phase shift is measured by an Ocean Optics spectrometer or MFPF system, and is related to the partial pressure of oxygen.
The FOSPOR coating is applied to the distal end of the fiber and has a fluorescence-based Pt-porphyrin indicator. The sensor uses fluorescence quenching of the porphyrin to measure the partial pressure of dissolved or gaseous oxygen. The FOSPOR coating can be applied to substrates other than fibers and probes and used for applications such as through-packaging measurements in the food and pharmaceutical industries. *Please contact an Applications Scientist before specifying FOSPOR or other coatings.
Two years of research and development pursuing a high-performance oxygen sensor compatible with hydrocarbon environments has resulted in our new HIOXY sensor. The HIOXY sensor is designed for monitoring oxygen partial pressure in non-aqueous vapors and solutions. The sensor coating chemistry is ideal for use with oils, alcohols and hydrocarbon-based vapors and liquids. HIOXY has been tested successfully in commercial and military aviation fuels, gasoline, diesel, some alcohols, glycol, military hydraulic fluids and wine analysis.
Once you choose the best Sensor Coating Formulation for your application, you need to select the fiber optic probe onto which the sensor coating is applied. For a list of fiber optic probes, click here.
| Specification | FOXY Probes | FOSPOR Probes | HIOXY Probes |
| Principle | Photoluminescence-quenching using Ru compound. Measures O2 partial pressure. | Photoluminescence-quenching using Pt compound. Measures O2 partial pressure. | Photoluminescence-quenching using Ru compound. Measures O2 partial pressure. |
| Sensor mechanism |
Phase shift due to change in partial pressure of O2 |
Phase shift due to change in partial pressure of O2 | Phase shift due to change in partial pressure of O2 |
| Applications | Benign environment, aqueous liquids and vapors | Low O2 (0–5%), vacuum systems packaging, benign environment | Hydrocarbon-based liquids and vapors, fuels, alcoholic beverages, vegetable oil |
| Time-constant (µsec) dynamic range | Air: 2.50, N2: 5.00; Low 1.00, High 7.00 | Air: 10.00; N2: 50.00; Low: 3.00, High: 70.00 | Air: 0.50, N2: 2.50; Low: 0.30, High: 6.00 |
| Accuracy: (0–20% O2, 0–50ºC) | 5% of reading (using polynomial fit to multipoint calibration) | 5% of reading (using polynomial fit to multipoint calibration) | 5% of reading (using polynomial fit to multipoint calibration) |
| Response time of probe with no overcoat | ~1 second, in liquid and gas | ~1 second, in liquid and gas | ~1 second, in liquid and gas |
| Response time of dissolved oxygen in liquid (with overcoat) | 30-45 seconds | 30-45 seconds | Overcoat not required |
| Response time of probe with Overcoat of Oxygen Gas (at 1 atmosphere) | 15-20 seconds | 15-20 seconds | Overcoat not required |
| Dynamic range of Dissolved Oxygen in liquid | 0–40.7 ppm; 0–760 mm Hg partial pressure | 0–40.7 ppm; 0–760 mm Hg partial pressure | 0–40.7 ppm; 0–760 mm Hg partial pressure |
| Dynamic range of Oxygen Gas (at 1 atmosphere) | 0–100% (mole percent); 0–760 mm Hg partial pressure | 0–100% (mole percent); 0–760 mm Hg partial pressure | 0–100% (mole percent); 0–760 mm Hg partial pressure |
| Stability Dissolved Oxygen in liquid | Drift<0.01 ppm per hour | Drift < 0.00008 ppm O2 per hour at low dissolved oxygen; Drift ~ 0.006 ppm O2 per hour in air-saturated water | Drift<0.002 ppm per hour |
| Stability Oxygen Gas (at 1 atmosphere) | Drift ~0.03% O2 per hour | Drift ~ 0.0002% O2 drift per hour at low O2; Drift ~ 0.015% O2 per hour air |
Drift ~0.01% O2 per hour
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| Resolution of Dissolved Oxygen in liquid | 0.02 ppm at room temperature | 0.002 ppm at room temperature | 0.02 ppm at room temperature |
| Resolution of Oxygen Gas (at 1 atmosphere) | 0.05% (0.4 mmHg) at room temperature | 0.005% at room temperature | 0.05% (0.4 mmHg) at room temperature |
| Lowest detectable limit of Dissolved Oxygen in liquid | 0.02 ppm | 0.002 ppm at room temperature | 0.02 ppm |
| Lowest detectable limit of oxygen gas (at 1 atmosphere) | 0.05% (0.4 mm Hg) | 0.005% (0.04 mm Hg) | 0.05% (0.4 mm Hg) |
| Overcoat available | Yes, order FOXY-AF or FOXY-AF-MG | Yes | No overcoat available |
| Temperature range | -50ºC to +80ºC | 0ºC to +60ºC | -50ºC to +80ºC |
| Temperature | Temperature sensor and software-supported compensation | Temperature sensor and software-supported compensation | Temperature sensor and software-supported compensation |
| Temperature recalibration | Only when probe is replaced/refurbished | Only when probe is replaced/refurbished | Only when probe is replaced/refurbished |
| Probe lifetime | Reconditioning recommended once per year | Reconditioning recommended once per year | Reconditioning recommended once per year |
Your Oxygen Sensor probe is very easy to maintain. It can be left in air indefinitely, but don't leave it exposed to your excitation light source when it is not in use. Dropping the probe could cause the optical fiber to break. Be sure not to over tighten the SMA connections.
Clean your probes with 10% hypochlorite detergent and sterilize them with Gamma radiation or sodium hypochlorite (bleach). See below for further details on cleaning and sterilization methods available for your Sensor Probes. *Please contact an Applications Scientist before specifying FOSPOR or other coatings.
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Cleaning Method |
FOXY Probes |
FOSPOR Probes |
HIOXY Probes |
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Sodium hypochlorite (bleach) |
Safe -- seems to have no effect on probes |
Safe -- seems to have no effect on probes |
Safe -- seems to have no effect on probes |
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Gamma radiation |
Safe -- seems to have no effect on probes |
Safe -- seems to have no effect on probes |
Safe -- seems to have no effect on probes |
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Hydrogen peroxide plasma gas, low temperature, Plazlyte |
Degrades probe signal by about 15% with each cycle |
Degrades probe signal by about 15% with each cycle |
Degrades probe signal by about 15% with each cycle |
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Autoclaving (steam sterilization) >30 minutes at 121 °C |
Each cycle decreases signal by 50%; probe lifetime is 6 to 8 cycles |
Each cycle decreases signal by 50%; probe lifetime is 6 to 8 cycles |
Each cycle decreases signal by 50%; probe lifetime is 6 to 8 cycles |
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Methanol and ethanol wash |
Destroys probe signal with extended exposure, even with overcoat |
Destroys probe signal with extended exposure, even with overcoat |
Safe with brief exposure to ethanol |
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Hydrogen peroxide |
Destroys probe signal with extended exposure, even with overcoat |
Destroys probe signal with extended exposure, even with overcoat |
Unknown |
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Ozone |
Preliminary results indicate that it is harmful to probes |
Preliminary results indicate that it is harmful to probes |
Preliminary results indicate that it is harmful to probes |
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EtO (100% ethylene oxide gas) at room temperature |
Unknown |
Unknown |
Unknown |
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EtO at temperatures above 100 °C |
Unknown |
Unknown |
Unknown |
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UV radiation |
Unknown |
Unknown |
Unknown |
The following tables lists known observational effects of chemicals and gases on Sensor Slides. If a chemical or gas passes the "observational" test (inserting the slide into the chemical/gas for a 24-hour period and observing no change in sensor performance), it warrants further comprehensive determinate testing. Please note that the table below lists compatibility on an observational level only.
Visual Observation of Sol Gel Coating on Slides
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Chemical / Gas |
FOXY and FOSPOR | HIOXY |
| Acetone (Reagent Plus, > 99%) | No | No |
| Acetonitrile | No | Yes |
| Acetonitrile Headspace | No | Yes |
| Acrylonitrile (99+ %) | No | No |
| Benzene (99%, AVS Reagent) | No | Yes |
| Cyclohexane | No | Yes |
| DichloroMethane | No | Yes |
| Diesel Fuel | No | Yes |
| Ethanol (Reagent, Denatured) | No | Yes |
| Gasoline | No | Yes |
| Glacial Acrylic Acid | No | No |
| Glacial Acetic Acid | No | No |
| Heptane | Yes | Yes |
| Hexane (95+%, HPLC grade) | Yes | Yes |
| Isopropyl Alcohol | No | Yes |
| Hydrogen Peroxide (3% H202 USP) | Yes | Yes |
| Methanol (98% Histological grade) | No | Yes |
| Methyl Methacrylate (99%) | Yes | No |
| Mineral Oil | No | Yes |
| Sodium Hypochlorite Solution (Reagent grade, available chlorine 10-13%) | No | No |
| Sodium Hydroxide | No | Yes |
| Sulfur Dioxide Solution Purris (PA 4.5-5.5% in H20) | Yes | Yes |
| Styrene (Reagent plus, > 99%) | No | Yes |
| Tetrahydrofuran (99+%) | No | No |
| Toluene (99%) | No | No |
| Xylene (Isomers plus ethyl benzene, Reagent plus) | No | Yes |
| Ethlyn Glycol | Yes | Yes |
| JP8 (Jet fuel) | No | Yes |
Probes Without Overcoat Immersed in Chemicals
| Chemical / Gas | FOXY and FOSPOR | HIOXY |
Comments |
| Acetone (Reagent Plus, > 99%) | No | No | Lifetime not reproducible |
| Acetonitrile | No | No | The lifetime of the probe fluctuates and is not stable in the solution |
| Acetonitrile Headspace | No | Yes | Needs additional conditioning in Acetonitrile headspace before use |
| Acrylonitrile (99+ %) | No | No | Lifetime not reproducible |
| Benzene (99%, AVS Reagent) | No | Yes | The probe has a one-time permanent decrease in lifetime when immersed in chemicals. Therefore, it needs to be calibrated in the chemical. |
| Cyclohexane | No | Yes | The Hioxy probe has a one-time permanent decrease in lifetime when immersed in chemicals. Therefore, it needs to be calibrated in the chemical. The Foxy probe has decreases in both signal level and lifetime. |
| DichloroMethane | No | Yes | The Hioxy probe has a one-time permanent decrease in lifetime when immersed in chemicals. Therefore, it needs to be calibrated in the chemical. The Foxy probe has decreases in both signal level and lifetime. |
| Diesel Fuel | No | Yes | |
| Ethanol (Reagent, Denatured) | No | Yes | The probe has a one-time permanent decrease in lifetime when immersed in chemicals. Therefore, it needs to be calibrated in the chemical. The probe needs to be cured in Ethanol for lifetime to stabilize. |
| Gasoline | No | Yes | |
| Glacial Acrylic Acid | No | No | |
| Glacial Acetic Acid | No | No | |
| Heptane | Yes | Yes | Foxy probes have a one-time permanent decrease in lifetime when immersed in chemicals. Therefore, it needs to be calibrated in the chemical. |
| Hexane (95+%, HPLC grade) | No | No | Lifetime not reproducible |
| Isopropyl Alcohol | No | Yes | The probe has a one-time permanent decrease in lifetime when immersed in chemicals. Therefore, it needs to be calibrated in the chemical. The signal level decreases every time the probe is immersed in ethanol and has a limited lifespan. |
| Hydrogen Peroxide (3% H202 USP) | No | Yes | Hioxy Probe- Signal level has a very small decay over a long period of time. Bubbles formed on the tip of the probe |
| Methanol (98% Histological grade) | No | Yes | The probe has a one-time permanent decrease in lifetime when immersed in chemicals. Therefore, it needs to be calibrated in the chemical. |
| Methyl Methacrylate (99%) | No | No | Lifetime not reproducible |
| Mineral Oil | No | Yes | The probe has a one-time permanent decrease in lifetime when immersed in chemicals. Therefore, it needs to be calibrated in the chemical. |
| Sodium Hypochlorite Solution (Reagent grade, available chlorine 10-13%) | No | No | Lifetime not reproducible |
| Sodium Hydroxide | No | No | Signal level and lifetime not reproducible |
| Sulfur Dioxide Solution Purris (PA 4.5-5.5% in H20) | No | No | Foxy Probe: Signal level in the chemical is low and has a large variation from 55 to 20 counts. The signal level and lifetime decrease each time the probe is dipped in the chemical. |
| Styrene (Reagent plus, > 99%) | No | Yes | Hioxy Probe: The probe has a one-time permanent decrease in lifetime when immersed in chemicals. Therefore, it needs to be calibrated in the chemical. |
| Tetrahydrofuran (99+%) | No | No | Lifetime not reproducible |
| Toluene (99%) | No | Yes | Hioxy Probe: The probe has a one-time permanent decrease in lifetime when immersed in chemicals. Therefore, it needs to be calibrated in the chemical. |
| Xylene (Isomers plus ethyl benzene, Reagent plus) | No | No | Lifetime not reproducible |
| Ethlyn Glycol | No | Yes | Hioxy Probe: The probe has a one-time permanent decrease in lifetime when immersed in chemicals. Therefore, it needs to be calibrated in the chemical. |
| JP8 (Jet fuel) | No | Yes |
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