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Fiber Optic Oxygen Sensors Neofox FOXY HIOXY and PHOSPOR


All Optical Oxygen Sensors!
Visit our new website ZeroDriftSensors.com for the latest on our exciting new chemistries. 

Probe styles and options
Sensor material options
Care and Chemical Compatibility

Fluorescence Quenching


This is the ultimate technology for measuring oxygen. We trap a fluorescent compound in an oxygen permeable, optically clear sol gel matrix. The fluoresence of this compound is excited by a blue LED. Molecular oxygen molecules collide with the excited state of the fluorophore and releases the enrgy non-radiatively. This queching effect is measured by phase fluorimetry as the average decay time, and is use to determine pO2 in the sensor. The sensor is only rendered impermeable to water vapor and other potential interferences by the sol gel chemistry. The result is a probe that only responds to the partial pressure of O2 and is not affected by turbidity, salinity, or other media effects. It works the same in gases as in liquids, and except for temperature is not affected by any other sources of drift exhobited by electrodes. The calibration is straightforward, as the quenching follows the Stern-Volmer quenching equation.

  • Fast (< 1 sec)
  • Dynamic Range
    • FOXY & HIOXY  0 - 100%
    • FOSPOR 0 - 5%
  • Sensitivity (gaseous)
    • FOXY & HIOXY 0.05%
    • FOSPOR 0.005%
  • Sensitivity (dissolved O2)
    • FOXY & HIOXY 0.02 ppm
    • FOSPOR 0.002ppm
  • Accurate (5% of reading)
  • No Oxygen Consumption
  • Responds only to pO2
  • Use in gases, liquids, slurries
  • Available as fiber probes or remotely sampled patches

Spectrecology sells sensors based on the finest materials in the world. Our sol-gel sensor materials are the culmination of over 15 years of R&D effort. No one makes fluorescent sensors with the stability and durability of our FOXY line. Originally developed for oceanographic use, these sensors are deployed in aircraft fuel tanks, bioreactors, chemical reactors, respitory therapy devices, food and pharmaceutical packaging plants, brain tisse, soil, tree sap and even CO2 lasers.

  •  Fiber Optic Oxygen Sensor Probe Options

    Fiber Optic Oxygen Sensors consist of an optical fiber polished to an angled surface and coated with a thin layer of sol-gel containing the active fluorophore. The probes can be made in many styles, depending on the requirement for size, ruggedness etc. If you don't find a style that meets your needs, ask us. We can easily build custome versions.

    Fiber Optic Oxygen Sensors are designed for use with our modular components. First select a Sensor Coating Formulation. Then select a Fiber Optic Probe onto which the sensor coating will be applied. The select an optical fiber and the NEOFOX electronics package of your choice. Need help? Just call us and we'll pick the best components for you.

    The tables below list various off-the-shelf probes and the reccomended bifurcated fiber assembly.

    R Sensor Probe

     Our standard lab probe is called the R probe. It is a 1/16" OD stainless steel ferrule with a 1000 um fiber.

     

     

    Probe Assembly: 1000 µm fiber in a stainless steel 1/16" OD ferrule
    Dimensions: 1.587 mm OD, 152.4 mm length
    Pressure: 300 psi
    Fiber: BIFBORO-1000-2
    Termination: SMA 905
    Typical Usage: general purpose

    AL300

    The AL300 is a 500 µm OD (300 µm core diameter) aluminum-jacketed optical fiber probe for applications that require fine spatial resolution.

    Probe Assembly: 300 µm aluminum-jacketed fiber assembly
    Dimensions: 500 µm OD, 1 m length
    Pressure: 300 psi
    Fiber: BIFBORO-300-2
    Termination: SMA 905
    Typical Usage: fine spatial resolution applications
    Price: $577

     

    18G

    The 18G is a 300 µm optical fiber housed in an 18-gauge stainless steel needle probe.
    *Refurbishing not available on this item

     

    Probe Assembly: 300 µm optical fiber, 18-gauge tip
    Dimensions: 1.27 mm diameter, 90 mm length tip
    Pressure: 300 psi
    Fiber: BIFBORO-300-2
    Termination: SMA 905
    Typical Usage: penetration of vial septa and rigid packaging
    Price:  $692

     

    21G

    The 21G is a 300 µm optical fiber housed in a 21-gauge stainless steel needle probe.
    *Refurbishing not available on this item

    Probe Assembly: 300 µm optical fiber, 21-gauge tip
    Dimensions: 813 µm diameter, 90 mm length tip
    Pressure: 300 psi
    Fiber: BIFBORO-300-2
    Termination: SMA 905
    Typical Usage: penetration of soft packaging
    Price:  $692

     

    OR125

    The OR125 is a 1/8" OD optical fiber probe used as a direct replacement for 1/8" OD O2 electrodes.

    Probe Assembly:

    1000 µm optical fiber, stainless steel ferrule

    Dimensions:

    3.175 mm OD, 63.5 mm length

    Pressure:

    300 psi

    Fiber:

    BIFBORO-1000-2

    Termination:

    SMA 905

    Typical Usage:

    direct replacement for 1/8" OD O2 electrodes

    Price: 

    $692

     

    OR125G

    The OR125 is a 1/8" OD optical fiber probe used as a direct replacement for 1/8" OD O2 electrodes.

    Probe Assembly: 1000 µm optical fiber, stainless steel ferrule
    Dimensions: 3.175 mm OD, 63.5 mm length
    Pressure: 300 psi
    Fiber: BIFBORO-1000-2
    Termination: SMA 905
    Typical Usage: direct replacement for O-ring grooved electrodes
    Price: $692

     

    OR125GT

    The OR125 is a 1/8" OD optical fiber probe used as a direct replacement for 1/8" OD O2 electrodes.

    Probe Assembly: 1000 µm optical fiber, titanium ferrule
    Dimensions: 3.175 mm OD, 63.5 mm length
    Pressure: 300 psi
    Fiber: BIFBORO-1000-2
    Termination: SMA 905
    Typical Usage: direct replacement for O-ring grooved electrodes
    Price: $751

     

    T1000

    The T1000 is a 6.35 mm OD optical fiber probe used in process environments and high-pressure applications.

    Probe Assembly: 1000 µm optical fiber, stainless steel ferrule
    Dimensions: 6.35 mm OD, 177.8 mm length
    Pressure: 3000 psi
    Fiber: BIFBORO-1000-2
    Termination: SMA 905
    Typical Usage: process environments, high-pressure applications
    Price: $1154

     

    T1000-RTD

    The T1000-RTD is a 6.35-mm OD optical fiber probe with an embedded RTD used in process environments. The T1000 RTD can only be used in conjunction with the USB-LS-450.

     

    Probe Assembly: 1000 µm optical fiber, 1/8" OD stainless steel
    Dimensions: 6.355 mm OD, 177.8 mm length
    Pressure: 300 psi
    Fiber: BIFBORO-1000-2
    Termination: SMA 905
    Typical Usage: process environments, includes embedded RTD
    Price: $1732

     

    PI600

    The PI600 is a silicone-jacketed, polyimide-coated optical fiber probe used in environments where a non-metallic probe is required.

    Probe Assembly: 600 µm optical fiber with silicone jacketing*
    Dimensions: 710 µm OD, 2 m length
    Pressure: 300 psi
    Fiber: BIFBORO-600-2
    Termination: SMA 905
    Typical Usage: environments where non-metallic probe is indicated
    Price: $577

    * 200 µm also available. Please call us for details.

    Optional Probe Silicone Overcoats

    Optional coatings of silicone (both standard and medical-grade) can be applied to Probes to exclude ambient light, improve chemical resistance and eliminate refractive index effects. We recommend an overcoat if your application involves liquids, as well as those that involve changing from gases to liquids, as scattering effects could result in the sensor losing calibration. There is no charge for these overcoats.

    Item Description Response in Gases Response in Liquids
    -AF RTV silicone adhesive overcoat (increases probe response time) 10-30 seconds 15-45 seconds
    -AF-MG High-strength RTV silicone adhesive overcoat is a medical implant-grade silicone 15-45 seconds 45-60 seconds (depending on sample viscosity)

     



Remote Detection!
-- That's right! We can sense the fluorescent decay time from a distance. Our Red Eye patches can be put on the inside of package, vial, respirometer, you name it. Point the fiber optic probe at the patch and get a fast accurate answer.

For details on O2 sensing patches, click here!

 

 Sol gel formulations for different applications...   The design of the fluorophore and the sol gel matrix is carefully controlled to optimize the coatings for specific purposes. Rutenium complexes are quenched over a broad range of pO2 levels and are excellent as general purpose probes. Pt complexes offer higher sensitivity over a narrow range of pO2 levels. The sol gel is made to exclude the various species that offer competitive quenching, and by doing this make the probes specific for O2. Our standard formulation is hydrophobic yet permeable to O2. Our HIOXY formulation excludes both polar and non polar molecules making it ideal for use in organic solvents.

FOXY Coating Formulation

Our Oxygen Sensing Probes with the FOXY Formulation applied are designed for monitoring partial pressure of oxygen in benign gasses and aqueous liquids. Standard FOXY probe tips are covered with a layer of hydrophobic sol-gel material with a ruthenium compound trapped in the sol-gel matrix. When excited by an LED, the ruthenium complex fluoresces. If the excited ruthenium complex then encounters an oxygen molecule, the excess energy quenches the fluorescent signal. The fluorescence intensity or phase shift is measured by an Ocean Optics spectrometer or MFPF system, and is related to the partial pressure of oxygen.

FOSPOR Coating Formulation

The FOSPOR coating is applied to the distal end of the fiber and has a fluorescence-based Pt-porphyrin indicator. The sensor uses fluorescence quenching of the porphyrin to measure the partial pressure of dissolved or gaseous oxygen. The FOSPOR coating can be applied to substrates other than fibers and probes and used for applications such as through-packaging measurements in the food and pharmaceutical industries. *Please contact an Applications Scientist before specifying FOSPOR or other coatings.

HIOXY Coating Formulation

Two years of research and development pursuing a high-performance oxygen sensor compatible with hydrocarbon environments has resulted in our new HIOXY sensor. The HIOXY sensor is designed for monitoring oxygen partial pressure in non-aqueous vapors and solutions. The sensor coating chemistry is ideal for use with oils, alcohols and hydrocarbon-based vapors and liquids. HIOXY has been tested successfully in commercial and military aviation fuels, gasoline, diesel, some alcohols, glycol, military hydraulic fluids and wine analysis.

Choosing a Probe

Once you choose the best Sensor Coating Formulation for your application, you need to select the fiber optic probe onto which the sensor coating is applied. For a list of fiber optic probes, click here.

Sensor Coating Specifications

Specification FOXY Probes FOSPOR Probes HIOXY Probes
Principle Photoluminescence-quenching using Ru compound. Measures O2 partial pressure. Photoluminescence-quenching using Pt compound. Measures O2 partial pressure. Photoluminescence-quenching using Ru compound. Measures O2 partial pressure.
Sensor mechanism

Phase shift due to change in partial pressure of O2

Phase shift due to change in partial pressure of O2 Phase shift due to change in partial pressure of O2
Applications Benign environment, aqueous liquids and vapors Low O2 (0–5%), vacuum systems packaging, benign environment Hydrocarbon-based liquids and vapors, fuels, alcoholic beverages, vegetable oil
Time-constant (µsec) dynamic range Air: 2.50, N2: 5.00; Low 1.00, High 7.00 Air: 10.00; N2: 50.00; Low: 3.00, High: 70.00 Air: 0.50, N2: 2.50; Low: 0.30, High: 6.00
Accuracy: (0–20% O2, 0–50ºC) 5% of reading (using polynomial fit to multipoint calibration) 5% of reading (using polynomial fit to multipoint calibration) 5% of reading (using polynomial fit to multipoint calibration)
Response time of probe with no overcoat ~1 second, in liquid and gas ~1 second, in liquid and gas ~1 second, in liquid and gas
Response time of dissolved oxygen in liquid (with overcoat) 30-45 seconds 30-45 seconds Overcoat not required
Response time of probe with Overcoat of Oxygen Gas (at 1 atmosphere) 15-20 seconds 15-20 seconds Overcoat not required
Dynamic range of Dissolved Oxygen in liquid 0–40.7 ppm; 0–760 mm Hg partial pressure 0–40.7 ppm; 0–760 mm Hg partial pressure 0–40.7 ppm; 0–760 mm Hg partial pressure
Dynamic range of Oxygen Gas (at 1 atmosphere) 0–100% (mole percent); 0–760 mm Hg partial pressure 0–100% (mole percent); 0–760 mm Hg partial pressure 0–100% (mole percent); 0–760 mm Hg partial pressure
Stability Dissolved Oxygen in liquid Drift<0.01 ppm per hour Drift < 0.00008 ppm O2 per hour at low dissolved oxygen; Drift ~ 0.006 ppm O2 per hour in air-saturated water Drift<0.002 ppm per hour
Stability Oxygen Gas (at 1 atmosphere) Drift ~0.03% O2 per hour Drift ~ 0.0002% O2 drift per hour at low O2; Drift ~ 0.015% O2 per hour air

Drift ~0.01% O2 per hour

 

Resolution of Dissolved Oxygen in liquid 0.02 ppm at room temperature 0.002 ppm at room temperature 0.02 ppm at room temperature
Resolution of Oxygen Gas (at 1 atmosphere) 0.05% (0.4 mmHg) at room temperature 0.005% at room temperature 0.05% (0.4 mmHg) at room temperature
Lowest detectable limit of Dissolved Oxygen in liquid 0.02 ppm 0.002 ppm at room temperature 0.02 ppm
Lowest detectable limit of oxygen gas (at 1 atmosphere) 0.05% (0.4 mm Hg) 0.005% (0.04 mm Hg) 0.05% (0.4 mm Hg)
Overcoat available Yes, order FOXY-AF or FOXY-AF-MG Yes No overcoat available
Temperature range -50ºC to +80ºC 0ºC to +60ºC -50ºC to +80ºC
Temperature Temperature sensor and software-supported compensation Temperature sensor and software-supported compensation Temperature sensor and software-supported compensation
Temperature recalibration Only when probe is replaced/refurbished Only when probe is replaced/refurbished Only when probe is replaced/refurbished
Probe lifetime Reconditioning recommended once per year Reconditioning recommended once per year Reconditioning recommended once per year
 


Care of Oxygen Sensor Probes

 Sterilization Method

Your Oxygen Sensor probe is very easy to maintain. It can be left in air indefinitely, but don't leave it exposed to your excitation light source when it is not in use. Dropping the probe could cause the optical fiber to break. Be sure not to over tighten the SMA connections.

Clean your probes with 10% hypochlorite detergent and sterilize them with Gamma radiation or sodium hypochlorite (bleach). See below for further details on cleaning and sterilization methods available for your Sensor Probes. *Please contact an Applications Scientist before specifying FOSPOR or other coatings.

Cleaning Method

FOXY Probes

FOSPOR Probes

HIOXY Probes

Sodium hypochlorite (bleach)

Safe -- seems to have no effect on probes

Safe -- seems to have no effect on probes

Safe -- seems to have no effect on probes

Gamma radiation

Safe -- seems to have no effect on probes

Safe -- seems to have no effect on probes

Safe -- seems to have no effect on probes

Hydrogen peroxide plasma gas, low temperature, Plazlyte

Degrades probe signal by about 15% with each cycle

Degrades probe signal by about 15% with each cycle

Degrades probe signal by about 15% with each cycle

Autoclaving (steam sterilization) >30 minutes at 121 °C

Each cycle decreases signal by 50%; probe lifetime is 6 to 8 cycles

Each cycle decreases signal by 50%; probe lifetime is 6 to 8 cycles

Each cycle decreases signal by 50%; probe lifetime is 6 to 8 cycles

Methanol and ethanol wash

Destroys probe signal with extended exposure, even with overcoat

Destroys probe signal with extended exposure, even with overcoat

Safe with brief exposure to ethanol

Hydrogen peroxide

Destroys probe signal with extended exposure, even with overcoat

Destroys probe signal with extended exposure, even with overcoat

Unknown

Ozone

Preliminary results indicate that it is harmful to probes

Preliminary results indicate that it is harmful to probes

Preliminary results indicate that it is harmful to probes

EtO (100% ethylene oxide gas) at room temperature

Unknown

Unknown

Unknown

EtO at temperatures above 100 °C

Unknown

Unknown

Unknown

UV radiation

Unknown

Unknown

Unknown

Compatibility
with Fiber Optic Oxygen Probes
: Observational Results

The following tables lists known observational effects of chemicals and gases on Sensor Slides. If a chemical or gas passes the "observational" test (inserting the slide into the chemical/gas for a 24-hour period and observing no change in sensor performance), it warrants further comprehensive determinate testing. Please note that the table below lists compatibility on an observational level only.

Visual Observation of Sol Gel Coating on Slides

Chemical / Gas

FOXY and FOSPOR HIOXY
Acetone (Reagent Plus, > 99%) No No
Acetonitrile No Yes
Acetonitrile Headspace No Yes
Acrylonitrile (99+ %) No No
Benzene (99%, AVS Reagent) No Yes
Cyclohexane No Yes
DichloroMethane No Yes
Diesel Fuel No Yes
Ethanol (Reagent, Denatured) No Yes
Gasoline No Yes
Glacial Acrylic Acid No No
Glacial Acetic Acid No No
Heptane Yes Yes
Hexane (95+%, HPLC grade) Yes Yes
Isopropyl Alcohol No Yes
Hydrogen Peroxide (3% H202 USP) Yes Yes
Methanol (98% Histological grade) No Yes
Methyl Methacrylate (99%) Yes No
Mineral Oil No Yes
Sodium Hypochlorite Solution (Reagent grade, available chlorine 10-13%) No No
Sodium Hydroxide No Yes
Sulfur Dioxide Solution Purris (PA 4.5-5.5% in H20) Yes Yes
Styrene (Reagent plus, > 99%) No Yes
Tetrahydrofuran (99+%) No No
Toluene (99%) No No
Xylene (Isomers plus ethyl benzene, Reagent plus) No Yes
Ethlyn Glycol Yes Yes
JP8 (Jet fuel) No Yes

Probes Without Overcoat Immersed in Chemicals

Chemical / Gas FOXY and FOSPOR HIOXY

Comments

Acetone (Reagent Plus, > 99%) No No Lifetime not reproducible
Acetonitrile No No The lifetime of the probe fluctuates and is not stable in the solution
Acetonitrile Headspace No Yes Needs additional conditioning in Acetonitrile headspace before use
Acrylonitrile (99+ %) No No Lifetime not reproducible
Benzene (99%, AVS Reagent) No Yes The probe has a one-time permanent decrease in lifetime when immersed in chemicals. Therefore, it needs to be calibrated in the chemical.
Cyclohexane No Yes The Hioxy probe has a one-time permanent decrease in lifetime when immersed in chemicals. Therefore, it needs to be calibrated in the chemical. The Foxy probe has decreases in both signal level and lifetime.
DichloroMethane No Yes The Hioxy probe has a one-time permanent decrease in lifetime when immersed in chemicals. Therefore, it needs to be calibrated in the chemical. The Foxy probe has decreases in both signal level and lifetime.
Diesel Fuel No Yes  
Ethanol (Reagent, Denatured) No Yes The probe has a one-time permanent decrease in lifetime when immersed in chemicals. Therefore, it needs to be calibrated in the chemical. The probe needs to be cured in Ethanol for lifetime to stabilize.
Gasoline No Yes  
Glacial Acrylic Acid No No  
Glacial Acetic Acid No No  
Heptane Yes Yes Foxy probes have a one-time permanent decrease in lifetime when immersed in chemicals. Therefore, it needs to be calibrated in the chemical.
Hexane (95+%, HPLC grade) No No Lifetime not reproducible
Isopropyl Alcohol No Yes The probe has a one-time permanent decrease in lifetime when immersed in chemicals. Therefore, it needs to be calibrated in the chemical. The signal level decreases every time the probe is immersed in ethanol and has a limited lifespan.
Hydrogen Peroxide (3% H202 USP) No Yes Hioxy Probe- Signal level has a very small decay over a long period of time. Bubbles formed on the tip of the probe
Methanol (98% Histological grade) No Yes The probe has a one-time permanent decrease in lifetime when immersed in chemicals. Therefore, it needs to be calibrated in the chemical.
Methyl Methacrylate (99%) No No Lifetime not reproducible
Mineral Oil No Yes The probe has a one-time permanent decrease in lifetime when immersed in chemicals. Therefore, it needs to be calibrated in the chemical.
Sodium Hypochlorite Solution (Reagent grade, available chlorine 10-13%) No No Lifetime not reproducible
Sodium Hydroxide No No Signal level and lifetime not reproducible
Sulfur Dioxide Solution Purris (PA 4.5-5.5% in H20) No No Foxy Probe: Signal level in the chemical is low and has a large variation from 55 to 20 counts. The signal level and lifetime decrease each time the probe is dipped in the chemical.
Styrene (Reagent plus, > 99%) No Yes Hioxy Probe: The probe has a one-time permanent decrease in lifetime when immersed in chemicals. Therefore, it needs to be calibrated in the chemical.
Tetrahydrofuran (99+%) No No Lifetime not reproducible
Toluene (99%) No Yes Hioxy Probe: The probe has a one-time permanent decrease in lifetime when immersed in chemicals. Therefore, it needs to be calibrated in the chemical.
Xylene (Isomers plus ethyl benzene, Reagent plus) No No Lifetime not reproducible
Ethlyn Glycol No Yes Hioxy Probe: The probe has a one-time permanent decrease in lifetime when immersed in chemicals. Therefore, it needs to be calibrated in the chemical.
JP8 (Jet fuel) No Yes  
 

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