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QE65000 cooled CCD Spectrometer

QE65000 back thinned cooled CCD array spectrometer features high quantum efficiency and low dark noi

The QE65000 Scientific-grade Spectrometer is a novel combination of detector, optical bench and electronics technologies that provides users with a remarkably sensitive system for demanding low-light level applications such as fluorescence, DNA sequencing, astronomy and Raman spectroscopy. The QE65000 can achieve up to 90% quantum efficiency with high signal-to-noise and rapid signal processing speed. The QE65000 Scientific-grade Spectrometer is available for $10499.

 

The QE65000 Scientific-grade Spectrometer is a novel combination of detector, optical bench and electronics technologies that provides users with a remarkably sensitive system for demanding low-light level applications such as fluorescence, DNA sequencing, astronomy and Raman spectroscopy. The QE65000 can achieve up to 90% quantum efficiency with high signal-to-noise and rapid signal processing speed. The QE65000 Scientific-grade Spectrometer is available in several versions:

Quantum Efficiency to 90%

The QE65000 is the most sensitive spectrometer we have ever developed. The Hamamatsu FFT-CCD detector used in the QE65000 provides 90% quantum efficiency (defined as how efficiently a photon is converted to a photo-electron). Most of our other detectors are linear CCDs but with this “2D” area detector, we can bin (or sum) a vertical row of pixels, which offers significant improvement in the signal-to-noise ratio (>1000:1) and signal processing speed of the detector compared with a linear CCD, where signals are digitally added by an external circuit.

Increased System Sensitivity

In our spectrometers with linear CCDs, the slit’s width, not its height, regulates the amount of light entering the bench because linear CCDs cannot efficiently collect the light from the entire height of the slit. But in the QE65000, the 2D area detector can better take advantage of the height of the slit and the additional light, greatly improving system sensitivity.

Excellent UV Response

Because the detector in the QE65000 is back-thinned (or back-illuminated), it has great native response in the UV and does not require the additional coatings that we typically apply to other detectors for UV applications.

Demanding Low Light-level Applications

The QE65000 Spectrometer is a great option for low-light level applications including fluorescence, Raman spectroscopy, DNA sequencing, astronomy and thin-film reflectivity. The TE-cooled (down to -15 °C) detector features low noise and low dark signal, which enables low-light-level detection and long integration times (analogous to the shutter speed of a camera) from 8 milliseconds to 15 minutes, with virtually no spectral distortion.


Onboard Programming

The QE65000 also has an onboard programmable micro-controller for controlling the spectrometer and accessories. You have access to 10 user-programmable digital inputs/outputs and a pulse generator for triggering other devices. You can use the I/Os to flash a lamp, stop/start a process, and send a message/alarm during the spectrometer’s integration period. The spectrometer’s operating parameters can be controlled through software. In fact, wavelength calibration coefficients unique to each spectrometer are programmed into a memory chip right on the spectrometer.

Plug-and-Play USB Operation

The QE65000 interfaces to a computer via USB 2.0 or RS-232 serial port. Data unique to each spectrometer are programmed into a memory chip on the QE65000; our Spectrasuite software reads these values for easy setup and hot swapping among computers, whether they run on Linux, Mac or Windows operating systems.

Optical Bench Options

At Ocean Optics, we understand that your work is unique and that you want a spectrometer to fit your specific needs. With the QE65000 Spectrometer, you can configure the bench for your application. Choose entrance aperture size, detector accessories, filters a grating and more. Our application scientists will help you determine which configuration will best work for you.

DNA and protein fluorescence detector

Physical

 

 

 

Dimensions:

 

 

 

182 mm x 110 mm x 47 mm

 

 

 

Weight:

 

 

 

1050 grams

 

 

 

Detector

 

 

 

Detector:

 

 

 

Hamamatsu S7031-1006

 

 

 

Detector range:

 

 

 

200-1100 nm

 

 

 

Pixels:

 

 

 

1024 x 58 (1044 x 64 total pixels); 24.6 µm square size

 

 

 

Pixel size:

 

 

 

24.576 µm square size

 

 

 

Pixel well depth:

 

 

 

300,000 electrons/well; ~ 1.5 million electrons/column

 

 

 

Sensitivity:

 

 

 

22 electrons/count for all wavelengths; 250 nm: 26 photons/count

 

 

 

Quantum efficiency:

 

 

 

90% peak

 

 

 

Optical Bench

 

 

 

Design:

 

 

 

f/4, Symmetrical crossed Czerny-Turner

 

 

 

Focal length:

 

 

 

101.6 mm input and output

 

 

 

Entrance aperture:

 

 

 

5, 10, 25, 50, 100 or 200 µm wide slits or fiber (no slit)

 

 

 

Grating options:

 

 

 

14 different grating options, UV through Shortwave NIR

 

 

 

HC-1 grating option:

 

 

 

provides 200-950 nm range

 

 

 

Detector collection lens option:

 

 

 

None

 

 

 

OFLV filter options:

 

 

 

OFLV-QE

 

 

 

Other bench filter options:

 

 

 

Longpass OF-1 filters

 

 

 

Collimating and focusing mirrors:

 

 

 

Standard only

 

 

 

UV enhanced window:

 

 

 

No

 

 

 

Fiber optic connector:

 

 

 

SMA 905 to 0.22 numerical aperture single-strand optical fiber

 

 

 

Spectroscopic

 

 

 

Wavelength range:

 

 

 

Grating dependent

 

 

 

Optical resolution:

 

 

 

~0.14-7.7 nm FWHM

 

 

 

Signal-to-noise ratio:

 

 

 

1000:1 (at full signal)

 

 

 

A/D resolution:

 

 

 

16 bit

 

 

 

Dark noise:

 

 

 

3 RMS counts

 

 

 

Dynamic range:

 

 

 

7.5 x 109 (system), 25000:1 for a single acquisition

 

 

 

Integration time:

 

 

 

8 ms to 15 minutes

 

 

 

Stray light:

 

 

 

<0.08% at 600 nm; 0.4% at 435 nm

 

 

 

Corrected linearity:

 

 

 

>99.8%

 

 

 

Electronics

 

 

 

Power consumption:

 

 

 

500 mA @ 5 VDC (no TE cooling); 3.5 A @ 5 VDC (with TE cooling)

 

 

 

Data transfer speed:

 

 

 

Full scans to memory every 7 ms with USB 2.0 port, 18 ms with USB1.1 port, 300 ms with serial port

 

 

 

Inputs/Outputs:

 

 

 

10 onboard digital user-programmable GPIOs (general purpose inputs/outputs)

 

 

 

Analog channels:

 

 

 

No

 

 

 

Auto nulling:

 

 

 

Yes

 

 

 

Breakout box compatibility:

 

 

 

Yes, HR4-BREAKOUT

 

 

 

Trigger modes:

 

 

 

4 modes

 

 

 

Strobe functions:

 

 

 

No

 

 

 

Gated delay feature:

 

 

 

Yes

 

 

 

Connector:

 

 

 

30-pin connector

 

 

 

Power-up time:

 

 

 

<5 seconds

 

 

 

Dark current:

 

 

 

4000 e-/pixel/sec @ 25 ºC; 200 e-/pixel/sec @ 0 ºC

 

 

 

Computer

 

 

 

Operating systems:

 

 

 

Windows 98/Me/2000/XP, Mac OS X and Linux with USB port; Any 32-bit Windows OS with serial port

 

 

 

Computer interfaces:

 

 

 

USB 2.0 @ 480 Mbps; RS-232 (2-wire) @ 115.2 K baud

 

 

 

Peripheral interfaces:

 

 

 

SPI (3-wire); I2C inter-integrated circuit

 

 

 

Temperature and Thermoelectric (TE) Cooling

 

 

 

Temperature limits:

 

 

 

-15 ºC to 50.0 ºC; no condensation

 

 

 

Set point:

 

 

 

Software controlled; lowest set point is 40 ºC below ambient, to -15 ºC

 

 

 

Stability

 

 

 

+/-0.1 ºC of set temperature in <2 minutes